I used  Meads Quarry, Island Home Ave, Knox Co. Tennessee Partial shade exposure Rock Quarry N35 57.162 W83 51.960 880 10/12/2014 to put into my microaquarium. I put water and dirt from this and added two plants. The plants added were:
Amblestegium varium (Hedwig) Lindberg. Moss.
Collection from: Natural spring. at Carters Mill Park, Carter Mill Road, Knox Co. TN. Partial shade exposure. N36 01.168 W83 42.832. 10/12/2014

Utricularia gibba L. Flowering plant. A
carnivorous plant. Original material from south shore of Spain Lake (N 35o55 12.35" W088o20' 47.00), Camp Bella Air Rd. East of Sparta Tn. in White Co. and grown in water tanks outside of greenhouse at Hesler
Biology Building. The University of Tennessee. Knox Co. Knoxville TN.
10/12/2014

 

After adding the plants, I observed the the organism in the water and there were nematodes swimming around and other organisms yet to be identified.